Heteroduplex Rejection in Yeast?
نویسندگان
چکیده
منابع مشابه
Heteroduplex rejection in yeast?
REPAIR of meiotically induced double-strand breaks duplex-rejection model. The danger of ignoring symmetric heteroduplexes was made especially relevant by (DSBs; Figure 1) is associated with a gradient of gene conversion, in which conversion frequency is high Hillers and Stahl’s (1999) evidence that symmetric heteroduplex does form in yeast (see the appendix, near the DSB site and falls with di...
متن کاملHeteroduplex DNA formation and homolog pairing in yeast meiotic mutants.
Previous studies of Saccharomyces cerevisiae have identified several meiosis-specific genes whose products are required for wild-type levels of meiotic recombination and for normal synaptonemal complex (SC) formation. Several of these mutants were examined in a physical assay designed to detect heteroduplex DNA (hDNA) intermediates in meiotic recombination. hDNA was not detected in the rec102, ...
متن کاملThe conversion gradient at HIS4 of Saccharomyces cerevisiae. I. Heteroduplex rejection and restoration of Mendelian segregation.
In Saccharomyces cerevisiae, some gene loci manifest gradients in the frequency of aberrant segregation in meiosis, with the high end of each gradient corresponding to a hotspot for DNA double-strand breaks (DSBs). The slope of a gradient is reduced when mismatch repair functions fail to act upon heteroduplex DNA-aberrant segregation frequencies at the low end of the gradient are higher in the ...
متن کاملHeteroduplex repair as an intermediate step of UV mutagenesis in yeast.
We have measured UV-induced mutation frequencies in yeast in a forward, nonselective assay system by scoring white adex ade2 double auxotrophs among parental red-pigmented ade2 clones. The frequencies of sectored and pure mutant clones were determined separately. In excision-defective strains carrying the genes rad1-1, rad3-2 and rad4-4, as well as in the double mutants, rad 1-1 rad 3-2 and rad...
متن کاملPatterns of heteroduplex formation associated with the initiation of meiotic recombination in the yeast Saccharomyces cerevisiae.
The double-strand break repair (DSBR) model of recombination predicts that heteroduplexes will be formed in regions that flank the double-strand break (DSB) site and that the resulting intermediate is resolved to generate either crossovers or noncrossovers for flanking markers. Previous studies in Saccharomyces cerevisiae, however, failed to detect heteroduplexes on both sides of the DSB site. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetics
سال: 2000
ISSN: 1943-2631
DOI: 10.1093/genetics/154.4.1913